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Joseph Mougous

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Joseph D. Mougous
Born1976
Alma materWestern Washington University (BS)
University of California, Berkeley (PhD)
AwardsBurroughs Wellcome Investigator in the Pathogenesis of Infectious Disease (2011)

NAS Award in Molecular Biology (2021)

Election to the National Academy of Sciences (2022)
Scientific career
InstitutionsUniversity of Washington
Websitehttps://mougouslab.org/

Joseph Mougous is an American microbiologist. He is a Professor of Microbiology, the Lynn M. and Michael D. Garvey Endowed Chair in Gastroenterology, the Director of the Microbial Interactions & Microbiome Center, and a Howard Hughes Medical Institute Investigator at the University of Washington.[1][2] Mougous is best known for contributions to the field interbacterial antagonism, including discovering the antibacterial activity of the Type VI secretion system (T6SS).[3][4]

Education

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Mougous graduated from Western Washington University with a BS in Chemistry in 1999. While there, he performed research in the laboratory of Professor David Patrick, using scanning tunnelling microscopy to study liquid crystals.[5] Mougous was recognized as the Outstanding Chemistry Graduate at Western Washington University in 1999.[6][7] He then pursued his Ph.D. at University of California-Berkeley under the mentorship of Carolyn Bertozzi, where he worked on the identification, characterization, and biosynthesis of sulfated metabolites in mycobacteria (including Mycobacterium tuberculosis).[8][9]

After receiving his PhD in 2004, Mougous joined the laboratory of John Mekalanos at Harvard Medical School as a Damon Runyon Postdoctoral Fellow.[10][11] There, he demonstrated that a previously uncharacterized gene cluster in Pseudomonas aeruginosa encodes a specialized protein secretion system (now referred to as a type VI secretion system, T6SS).[12]

Research and career

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Mougous joined the faculty as an Assistant Professor in the Department of Microbiology at the University of Washington in 2008. He is currently the Lynn M. and Michael D. Garvey Endowed Chair in Gastroenterology and the Director of the Microbial Interactions & Microbiome Center at the University of Washington.[2]

In 2010, Mougous’ laboratory reported that the T6SS of P. aeruginosa targets toxins to other bacterial cells.[3] Subsequently, Mougous and co-workers put forth the hypothesis that interbacterial antagonism is a general feature of the T6SS,[13] and the Mougous lab was the first to demonstrate interspecies targeting by the T6SS.[13] His group was also first to identify the molecular target of a T6SS antibacterial toxin, peptidoglycan, and they went on to identify numerous additional molecular targets of T6SS toxin superfamilies.[14][15][16][17][18] The Mougous lab also contributed early findings supporting the concept that the T6SS helps shape the composition of human-associated microbial communities.[19][20] In 2020, the Mougous group published a collaborative study with David R. Liu of Harvard University in which they repurposed a mutagenic antibacterial toxin from Burkholderia, DddA, into a genome editing tool designed for Mitochondrial DNA.[21]

In addition to work on the T6SS, the Mougous lab demonstrated that the Esx pathway (also known as the Type VII secretion system) functions analogously to the T6SS in delivering toxins to competing bacterial cells.[22] Additionally, they described the genetic engineering of an epibiont from the Patescibacteria phylum (also known as the Candidate phyla radiation).[23][24][25]

Honors and awards

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  • 1999    Outstanding Chemistry Graduate, Western Washington University, Bellingham, WA[6]
  • 2000    Ford Foundation Predoctoral Fellow, University of California, Berkeley, CA[26]
  • 2005    Damon Runyon Postdoctoral Fellow, Harvard Medical School, Boston, MA[11]
  • 2011    Burroughs Wellcome Investigator in the Pathogenesis of Infectious Disease, University of Washington, Seattle, WA[27]
  • 2011    Irving S. Sigal Memorial Award, American Society for Microbiology[28]
  • 2015    Investigator, Howard Hughes Medical Institute[29]
  • 2018    Finalist, Blavatnik Awards for Young Scientists[30]
  • 2019    Finalist, Blavatnik Awards for Young Scientists[30]
  • 2021    NAS Award in Molecular Biology[31]
  • 2022    Election to the National Academy of Sciences[32]

References

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  1. ^ "Joseph D. Mougous | UW Microbiology". microbiology.washington.edu. Retrieved 2024-02-28.
  2. ^ a b "About". Microbial Interactions & Microbiome Center. Retrieved 2024-02-29.
  3. ^ a b Hood, Rachel D.; Singh, Pragya; Hsu, FoSheng; Güvener, Tüzün; Carl, Mike A.; Trinidad, Rex R.S.; Silverman, Julie M.; Ohlson, Brooks B.; Hicks, Kevin G.; Plemel, Rachael L.; Li, Mo; Schwarz, Sandra; Wang, Wenzhuo Y.; Merz, Alexey J.; Goodlett, David R.; Mougous, Joseph D. (January 2010). "A Type VI Secretion System of Pseudomonas aeruginosa Targets a Toxin to Bacteria". Cell Host & Microbe. 7 (1): 25–37. doi:10.1016/j.chom.2009.12.007. PMC 2831478. PMID 20114026.[non-primary source needed]
  4. ^ Hood, Rachel D.; Peterson, S. Brook; Mougous, Joseph D. (2017-03-08). "From Striking Out to Striking Gold: Discovering that Type VI Secretion Targets Bacteria". Cell Host & Microbe. 21 (3): 286–289. doi:10.1016/j.chom.2017.02.001. ISSN 1934-6069. PMC 6404758. PMID 28279332.
  5. ^ Mougous, Joseph D.; Brackley, Andrew J.; Foland, Katherine; Baker, Robert T.; Patrick, David L. (20 March 2000). "Formation of Uniaxial Molecular Films by Liquid-Crystal Imprinting in a Magnetic Field". Physical Review Letters. 84 (12): 2742–2745. Bibcode:2000PhRvL..84.2742M. doi:10.1103/PhysRevLett.84.2742. PMID 11017314.[non-primary source needed]
  6. ^ a b Hougen, Denice, ed. (Fall 1999). "Chemical Vibrations Annual Newsletter #2 Fall 1999" (PDF). Chemical Vibrations: Annual Newsletter to Alumni and Friends of the Department.
  7. ^ Sheehan, Kathy (Fall 1998). "Window on Western, 1998, Volume 05, Issue 01". Western Washington University Western CEDAR. Retrieved February 28, 2024.
  8. ^ Mougous, Joseph David (2004). The role of sulfated molecules in Mycobacterium tuberculosis pathogenesis (Thesis). ProQuest 305210506.[page needed][non-primary source needed]
  9. ^ "People". Bertozzi Group. Retrieved 2024-02-28.
  10. ^ "Alumni - Mekalanos Lab". mekalanoslab.med.harvard.edu. Retrieved 2024-02-28.
  11. ^ a b "Eight Damon Runyon alumni elected to the National Academy of Sciences". Damon Runyon. Retrieved 2024-02-29.
  12. ^ Mougous, Joseph D.; Cuff, Marianne E.; Raunser, Stefan; Shen, Aimee; Zhou, Min; Gifford, Casey A.; Goodman, Andrew L.; Joachimiak, Grazyna; Ordoñez, Claudia L.; Lory, Stephen; Walz, Thomas; Joachimiak, Andrzej; Mekalanos, John J. (9 June 2006). "A Virulence Locus of Pseudomonas aeruginosa Encodes a Protein Secretion Apparatus". Science. 312 (5779): 1526–1530. Bibcode:2006Sci...312.1526M. doi:10.1126/science.1128393. PMC 2800167. PMID 16763151.[non-primary source needed]
  13. ^ a b Schwarz, Sandra; Hood, Rachel D.; Mougous, Joseph D. (December 2010). "What is type VI secretion doing in all those bugs?". Trends in Microbiology. 18 (12): 531–537. doi:10.1016/j.tim.2010.09.001. PMC 2991376. PMID 20961764.[non-primary source needed]
  14. ^ Russell, Alistair B.; Hood, Rachel D.; Bui, Nhat Khai; LeRoux, Michele; Vollmer, Waldemar; Mougous, Joseph D. (2011). "Type VI secretion delivers bacteriolytic effectors to target cells". Nature. 475 (7356): 343–347. doi:10.1038/nature10244. PMC 3146020. PMID 21776080.[non-primary source needed]
  15. ^ Russell, Alistair B.; Singh, Pragya; Brittnacher, Mitchell; Bui, Nhat Khai; Hood, Rachel D.; Carl, Mike A.; Agnello, Danielle M.; Schwarz, Sandra; Goodlett, David R.; Vollmer, Waldemar; Mougous, Joseph D. (May 2012). "A Widespread Bacterial Type VI Secretion Effector Superfamily Identified Using a Heuristic Approach". Cell Host & Microbe. 11 (5): 538–549. doi:10.1016/j.chom.2012.04.007. PMC 3358704. PMID 22607806.[non-primary source needed]
  16. ^ Russell, Alistair B.; LeRoux, Michele; Hathazi, Krisztina; Agnello, Danielle M.; Ishikawa, Takahiko; Wiggins, Paul A.; Wai, Sun Nyunt; Mougous, Joseph D. (April 2013). "Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors". Nature. 496 (7446): 508–512. Bibcode:2013Natur.496..508R. doi:10.1038/nature12074. PMC 3652678. PMID 23552891.[non-primary source needed]
  17. ^ Whitney, John C.; Quentin, Dennis; Sawai, Shin; LeRoux, Michele; Harding, Brittany N.; Ledvina, Hannah E.; Tran, Bao Q.; Robinson, Howard; Goo, Young Ah; Goodlett, David R.; Raunser, Stefan; Mougous, Joseph D. (October 2015). "An Interbacterial NAD(P)+ Glycohydrolase Toxin Requires Elongation Factor Tu for Delivery to Target Cells". Cell. 163 (3): 607–619. doi:10.1016/j.cell.2015.09.027. PMC 4624332. PMID 26456113.[non-primary source needed]
  18. ^ Ting, See-Yeun; Bosch, Dustin E.; Mangiameli, Sarah M.; Radey, Matthew C.; Huang, Shuo; Park, Young-Jun; Kelly, Katherine A.; Filip, Szymon Krzysztof; Goo, Young Ah; Eng, Jimmy K.; Allaire, Marc; Veesler, David; Wiggins, Paul A.; Peterson, S. Brook; Mougous, Joseph D. (November 2018). "Bifunctional Immunity Proteins Protect Bacteria against FtsZ-Targeting ADP-Ribosylating Toxins". Cell. 175 (5): 1380–1392.e14. doi:10.1016/j.cell.2018.09.037. PMC 6239978. PMID 30343895.[non-primary source needed]
  19. ^ Ross, Benjamin D.; Verster, Adrian J.; Radey, Matthew C.; Schmidtke, Danica T.; Pope, Christopher E.; Hoffman, Lucas R.; Hajjar, Adeline M.; Peterson, S. Brook; Borenstein, Elhanan; Mougous, Joseph D. (2019). "Human gut bacteria contain acquired interbacterial defence systems". Nature. 575 (7781): 224–228. Bibcode:2019Natur.575..224R. doi:10.1038/s41586-019-1708-z. PMC 6938237. PMID 31666699.[non-primary source needed]
  20. ^ Verster, Adrian J.; Ross, Benjamin D.; Radey, Matthew C.; Bao, Yiqiao; Goodman, Andrew L.; Mougous, Joseph D.; Borenstein, Elhanan (September 2017). "The Landscape of Type VI Secretion across Human Gut Microbiomes Reveals Its Role in Community Composition". Cell Host & Microbe. 22 (3): 411–419.e4. doi:10.1016/j.chom.2017.08.010. PMC 5679258. PMID 28910638.[non-primary source needed]
  21. ^ Mok, Beverly Y.; de Moraes, Marcos H.; Zeng, Jun; Bosch, Dustin E.; Kotrys, Anna V.; Raguram, Aditya; Hsu, FoSheng; Radey, Matthew C.; Peterson, S. Brook; Mootha, Vamsi K.; Mougous, Joseph D.; Liu, David R. (2020). "A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing". Nature. 583 (7817): 631–637. Bibcode:2020Natur.583..631M. doi:10.1038/s41586-020-2477-4. PMC 7381381. PMID 32641830.[non-primary source needed]
  22. ^ Whitney, John C; Peterson, S Brook; Kim, Jungyun; Pazos, Manuel; Verster, Adrian J; Radey, Matthew C; Kulasekara, Hemantha D; Ching, Mary Q; Bullen, Nathan P; Bryant, Diane; Goo, Young Ah; Surette, Michael G; Borenstein, Elhanan; Vollmer, Waldemar; Mougous, Joseph D (11 July 2017). "A broadly distributed toxin family mediates contact-dependent antagonism between gram-positive bacteria". eLife. 6. doi:10.7554/eLife.26938. PMC 5555719. PMID 28696203.[non-primary source needed]
  23. ^ Wang, Yaxi; Gallagher, Larry A.; Andrade, Pia A.; Liu, Andi; Humphreys, Ian R.; Turkarslan, Serdar; Cutler, Kevin J.; Arrieta-Ortiz, Mario L.; Li, Yaqiao; Radey, Matthew C.; McLean, Jeffrey S.; Cong, Qian; Baker, David; Baliga, Nitin S.; Peterson, S. Brook; Mougous, Joseph D. (October 2023). "Genetic manipulation of Patescibacteria provides mechanistic insights into microbial dark matter and the epibiotic lifestyle". Cell. 186 (22): 4803–4817.e13. doi:10.1016/j.cell.2023.08.017. PMC 10633639. PMID 37683634.[non-primary source needed]
  24. ^ Learn, Joshua Rapp (23 October 2023). "Diving Deep into the Mouth's Microbial Dark Matter". Discover Magazine.
  25. ^ "Genetic tools probe microbial dark matter". ScienceDaily (Press release). University of Washington School of Medicine/UW Medicine. 7 September 2023.
  26. ^ "Fellow Detail". ra.nas.edu. Retrieved 2024-02-28.
  27. ^ "Grant Recipients". Burroughs Wellcome Fund. Retrieved 2024-02-29.
  28. ^ "Science X Network :: Phys.org, Medical Xpress, Tech Xplore". sciencex.com. Retrieved 2024-02-29.
  29. ^ "Joseph D. Mougous, PhD | Investigator Profile | 2015-Present". www.hhmi.org. Retrieved 2024-02-29.
  30. ^ a b "National Finalists | Blavatnik Awards for Young Scientists". blavatnikawards.org. Retrieved 2024-03-11.
  31. ^ "NAS Award in Molecular Biology". www.nasonline.org. Retrieved 2024-03-11.
  32. ^ "Four UW researchers elected to the National Academy of Sciences for 2022". UW News. Retrieved 2024-02-29.
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